{"id":2461,"date":"2022-09-05T15:02:28","date_gmt":"2022-09-05T07:02:28","guid":{"rendered":"http:\/\/www.gongyeqx.com\/?p=2461"},"modified":"2022-09-05T15:23:39","modified_gmt":"2022-09-05T07:23:39","slug":"cw003-hsf95-%e6%98%86%e8%99%ab%e5%9f%b9%e5%85%bb%e5%9f%ba%e5%8f%af%e6%9b%bf%e6%8d%a2","status":"publish","type":"post","link":"http:\/\/www.gongyeqx.com\/?p=2461","title":{"rendered":"CW003 HSF95 \u6606\u866b\u57f9\u517b\u57fa\u53ef\u66ff\u6362"},"content":{"rendered":"

\u4ea7\u54c1\u63cf\u8ff0<\/span><\/p>\n

CW003\u57f9\u517b\u57fa\u9002\u7528\u4e8esf9\u3001High Five\u7b49\u6606\u866b\u7ec6\u80de\u60ac\u6d6e\u57f9\u517b\u548c\u77ac\u65f6\u8868\u8fbe\uff0c\u542b\u6709\u7ec6\u80de\u751f\u957f\u7684\u5168<\/p>\n

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\u4ea7\u54c1\u540d\u79f0\uff1aHSF95 Insect Cell Medium<\/p>\n

\u4ea7\u54c1\u8d27\u53f7\uff1a<\/span>CW003<\/p>\n

\u4ea7\u54c1\u89c4\u683c\uff1a<\/span>1L<\/p>\n

\u4ea7\u54c1\u7279\u70b9<\/span><\/p>\n

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\uf0d8 \u652f\u6301\u6746\u72b6\u75c5\u6bd2\u8f7d\u4f53\u8868\u8fbe\u7cfb\u7edf\u751f\u4ea7\u91cd\u7ec4\u57fa\u56e0\u86cb\u767d<\/p>\n

\n

\u57fa\u7840\u53c2\u6570<\/span><\/p>\n

\u7f16\u8f91<\/p>\n

\n

\u4ea7\u54c1\u4f7f\u7528\u65b9\u6cd5<\/span><\/p>\n

\u7ec6\u80de\u9a6f\u5316\u4e0e\u4f20\u4ee3<\/p>\n

1\uff09 \u57f9\u517b\u6761\u4ef6<\/p>\n

\u6e29\u5ea6\uff1a27oC\uff1b\u63a8\u8350\u6447\u5e8a\u8f6c\u901f\uff1a110-130 rpm\uff1b\u4f20\u4ee3\u7ec6\u80de\u5bc6\u5ea6\u63a7\u5236\u57280.8-1.2\u00d7106 cells\/mL\u3002<\/p>\n

2\uff09 \u76f4\u63a5\u9a6f\u5316<\/p>\n

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\u57fa\uff0c\u53ea\u8981\u6309\u7167\u65e5\u5e38\u4f20\u4ee3\u65b9\u5f0f\uff08\u7a00\u91ca\uff09\u66f4\u6362\u57f9\u517b\u57fa\u5373\u53ef\u3002<\/p>\n

3\uff09 \u6e10\u8fdb\u5f0f\u9a6f\u5316<\/p>\n

\u6ce8\u610f\uff1a\u9009\u7528\u4f4e\u4ee3\u6b21\u3001\u5904\u4e8e\u5bf9\u6570\u751f\u7684\u7ec6\u80de\u8fdb\u884c\u9a6f\u5316\u3002<\/p>\n

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+CW003\u57f9\u517b\u57fa\uff08CW003\u6bd4\u4f8b\u4ece25%\u81f390%\uff09\u9010\u6e10\u9a6f\u5316\uff0c\u4f20\u4ee3\u7ec6\u80de\u5bc6\u5ea6\u63a7\u5236\u57280.8-1.1\u00d7106<\/p>\n

cells\/mL\uff0c\u6bcf48h\u8fdb\u884c\u4f20\u4ee3\u3002<\/p>\n

\u2461\u5f85\u7ec6\u80de\u5728\u542b90%\u4ee5\u4e0aCW003\u57f9\u517b\u57fa\u751f\u957f\u6b63\u5e38\u540e\uff0c\u53ef\u5c1d\u8bd5\u5c06\u7ec6\u80de\u4f20\u4ee3\u81f3100% CW003\u57f9\u517b\u57fa<\/p>\n

\u4e2d\uff0c\u5e76\u8fde\u7eed\u4f20\u4ee33-4\u6b21\uff0c\u5982\u7ec6\u80de\u4fdd\u6301\u7a33\u5b9a\u751f\u957f\uff0c\u5373\u5df2\u9002\u5e94\u81f3CW003\u57f9\u517b\u57fa\u3002<\/p>\n

\u7ec6\u80de\u590d\u82cf<\/p>\n

1\uff09 \u8fc5\u901f\u53d6\u51fa\u51bb\u5b58\u7684\u7ec6\u80de\uff0c\u572837\u2103\u6c34\u6d74\u6761\u4ef6\u4e0b\u8fdb\u884c\u5feb\u901f\u89e3\u51bb\uff08\u53ef\u4ee5\u5728\u6c34\u4e2d\u8f7b\u8f7b\u6643\u52a8\uff0c\u65f6\u95f4\u63a7\u5236<\/p>\n

\u5728120s\u4ee5\u5185\uff09\uff0c\u81f3\u51bb\u5b58\u7ba1\u5185\u5269\u4f59\u5c0f\u5757\u51b0\u6676\u3002<\/p>\n

2\uff09 \u5728\u751f\u7269\u5b89\u5168\u67dc\u4e2d\uff0c\u5c06\u878d\u5316\u7684\u7ec6\u80de\u5168\u90e8\u8f6c\u79fb\u81f3\u65e0\u83cc\u79bb\u5fc3\u7ba1\u4e2d\uff0c\u52a0\u516510 mL\u9884\u70ed\u81f337\u2103\u7684\u57f9\u517b<\/p>\n

\u57fa\uff0c\u79bb\u5fc3\u6362\u6db2\uff08175 g\uff0c5 min\uff09\u53bb\u9664\u5168\u90e8\u4e0a\u6e05\uff0c\u52a0\u516520-30mL\u9884\u70ed\u81f337\u2103\u65b0\u9c9c\u57f9\u517b\u57fa\u91cd\u60ac\uff0c\u540e<\/p>\n

\u8f6c\u79fb\u81f3\u6447\u74f6\u57f9\u517b\u3002<\/p>\n

\u7ec6\u80de\u51bb\u5b58<\/p>\n

1\uff09 \u51bb\u5b58\u7ec6\u80de\u65f6\uff0c\u8981\u9009\u62e9\u5904\u4e8e\u5bf9\u6570\u751f\u3001\u72b6\u6001\u826f\u597d\uff0c\u6d3b\u529b\u226598%\u7684\u7684\u7ec6\u80de\uff1b<\/p>\n

2\uff09 \u4e8b\u5148\u8ba1\u7b97\u597d\u51bb\u5b58\u7684\u7ec6\u80de\u7ba1\u6570\u548c\u6240\u7528\u7684\u57f9\u517b\u57fa\u7684\u4f53\u79ef\uff0c\u51bb\u5b58\u5bc6\u5ea6\u4e3a2.5-3.5\u00d7107 \uff08\u901a\u5e38\u51bb\u5b58<\/p>\n

\u6d53\u5ea6\u4e3a1.0-2.0\u00d7107\uff09cells\/ml\/\u652f\uff1b<\/p>\n

3\uff09 \u7ec6\u80de\u51bb\u5b58\u6db2\u914d\u5236\uff1a<\/p>\n

45%\u65b0\u9c9c\u57f9\u517b\u57fa+45%\u539f\u57f9\u517b\u57fa\uff08\u4e0a\u6e05\uff09+10%DMSO\uff0c\u5145\u5206\u6df7\u5300\uff0c\u5b58\u653e\u4e8e4\u2103\uff0c\u4e00\u822c\u4e3a\u5f53<\/p>\n

\u5929\u7528\u5f53\u5929\u5236\u5907\uff1b<\/p>\n

4\uff09 \u4ee5175 g\uff0c5 min\u7684\u6761\u4ef6\u79bb\u5fc3\u6536\u96c6\u7ec6\u80de\uff0c\u5f03\u4e0a\u6e05\uff0c\u7528\u51bb\u5b58\u57f9\u517b\u57fa\uff08<\/p>\n

4\u2103 \uff09\u91cd\u60ac\u7ec6\u80de\uff1b<\/p>\n

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6\uff09 \u5c06\u7ec6\u80de\u51bb\u5b58\u7ba1\u653e\u5230\u7a0b\u5e8f\u964d\u6e29\u51bb\u5b58\u76d2\uff08\u6ce8\u610f\u586b\u5145\u5f02\u4e19\u9187\uff0c4\u2103\u9884\u51b7\uff09\u4e2d\uff0c\u7f6e\u4e8e-80\u2103 \u51b0\u7bb1(\u6bcf<\/p>\n

\u5206\u949f\u4e0b\u964d1\u2103)\uff0c\u8fc7\u591c\u5b58\u653e\u540e\u5c06\u7ec6\u80de\u8f6c\u5165\u6db2\u6c2e\u7f50\u4e2d\u8fdb\u884c\u4fdd\u5b58\u3002<\/p>\n<\/article>\n

<\/p>\n

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