{"id":2470,"date":"2022-11-13T15:22:00","date_gmt":"2022-11-13T15:22:00","guid":{"rendered":"http:\/\/www.gongyeqx.com\/?p=2470"},"modified":"2022-09-05T15:23:16","modified_gmt":"2022-09-05T07:23:16","slug":"rna%e8%b4%a8%e9%87%8f%e6%a3%80%e6%b5%8b","status":"publish","type":"post","link":"http:\/\/www.gongyeqx.com\/?p=2470","title":{"rendered":"RNA\u8d28\u91cf\u68c0\u6d4b"},"content":{"rendered":"<p><\/p>\n<p><span>\u745e\u6c0f\u7ee6\u866b\u901a\u7528\u63a2\u9488\u6cd5\u8367\u5149\u5b9a\u91cfPCR\u8bd5\u5242\u76d2\u4ef7\u683c1\uff09\u7d2b\u5916\u5438\u6536\u6cd5\u6d4b\u5b9a<\/span><\/p>\n<p><span>\u5148\u7528\u7a00\u91ca\u7528\u7684TE\u6eb6\u6db2\u5c06\u5206\u5149\u5149\u5ea6\u8ba1\u8c03\u96f6\u3002\u7136\u540e\u53d6\u5c11\u91cfRNA\u6eb6\u6db2\u7528TE\u7a00\u91ca\uff081:100\uff09\u540e\uff0c\u8bfb\u53d6\u5176\u5728\u5206\u5149\u5149\u5ea6\u8ba1260nm\u548c280nm\u5904\u7684\u5438\u6536\u503c\uff0c\u6d4b\u5b9aRNA\u6eb6\u6db2 \u6d53\u5ea6\u548c\u7eaf\u5ea6\u3002<\/span><\/p>\n<p><span>\u6d53\u5ea6\u6d4b\u5b9a<\/span><\/p>\n<p><span>A260\u4e0b\u8bfb\u503c\u4e3a1\u8868\u793a40 g RNA\/ml\u3002\u6837\u54c1RNA\u6d53\u5ea6(g\/ml)\u8ba1\u7b97\u516c\u5f0f\u4e3a\uff1aA260 \u00d7\u7a00\u91ca\u500d\u6570\u00d7 40 g\/ml\u3002\u5177\u4f53\u8ba1\u7b97\u5982\u4e0b\uff1a<\/span><\/p>\n<p><span>RNA\u6eb6\u4e8e40 l DEPC\u6c34\u4e2d\uff0c\u53d65ul\uff0c1:100\u7a00\u91ca\u81f3495\u7684TE\u4e2d\uff0c\u6d4b\u5f97A260 = 0.21<\/span><\/p>\n<p><span>RNA \u6d53\u5ea6= 0.21 \u00d7100 \u00d740 g\/ml = 840 g\/ml \u6216 0.84 g<\/span><\/p>\n<p><span>\u53d65ul\u7528\u6765\u6d4b\u91cf\u4ee5\u540e\uff0c\u5269\u4f59\u6837\u54c1RNA\u4e3a35 l\uff0c\u5269\u4f59RNA\u603b\u91cf\u4e3a\uff1a<\/span><\/p>\n<p><span>35 l \u00d7 0.84 gl = 29.4 g<\/span><\/p>\n<p><span>\u2461\u7eaf\u5ea6\u68c0\u6d4b<\/span><\/p>\n<p><span>RNA\u6eb6\u6db2\u7684A260\/A280\u7684\u6bd4\u503c\u5373\u4e3aRNA\u7eaf\u5ea6\uff0c\u6bd4\u503c\u8303\u56f41.8\u52302.1\u3002<\/span><\/p>\n<p><span>2\uff09\u53d8\u6027\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u6d4b\u5b9a<\/span><\/p>\n<p><span>\u2460\u5236\u80f6<\/span><\/p>\n<p><span>1g\u743c\u8102\u7cd6\u6eb6\u4e8e72ml\u6c34\u4e2d\uff0c\u51b7\u5374\u81f360\u2103\uff0c10 ml\u768410\u00d7 MOPS\u7535\u6cf3\u7f13\u51b2\u6db2\u548c18 ml\u768437% \u7532\u919b\u6eb6\u6db2(12.3 M)\u3002<\/span><\/p>\n<p><span>10\u00d7MOPS\u7535\u6cf3\u7f13\u51b2\u6db2<\/span><\/p>\n<p><span>\u6d53\u5ea6 \u6210\u5206<\/span><\/p>\n<p><span>0.4M MOPS\uff0cpH 7.0<\/span><\/p>\n<p><span>0.1M \u4e59\u9178\u94a0<\/span><\/p>\n<p><span>0.01M EDTA<\/span><\/p>\n<p><span>\u704c\u5236\u51dd\u80f6\u677f\uff0c\u9884\u7559\u52a0\u6837\u5b54\u81f3\u5c11\u53ef\u4ee5\u52a0\u516525 l\u6eb6\u6db2\u3002\u80f6\u51dd\u540e\u53d6\u4e0b\u68b3\u5b50\uff0c\u5c06\u51dd\u80f6\u677f\u653e\u5165\u7535\u6cf3\u69fd\u5185\uff0c\u52a0\u8db3\u91cf\u76841\u00d7MOPS\u7535\u6cf3\u7f13\u51b2\u6db2\u81f3\u8986\u76d6\u80f6\u9762\u51e0\u4e2a\u6beb\u7c73\u3002<\/span><\/p>\n<p><\/p>\n<\/p><\/div>\n<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"<p class=\"qua-blog-post-description\">[db:\u6458\u8981]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[4],"tags":[5],"_links":{"self":[{"href":"http:\/\/www.gongyeqx.com\/index.php?rest_route=\/wp\/v2\/posts\/2470"}],"collection":[{"href":"http:\/\/www.gongyeqx.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.gongyeqx.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.gongyeqx.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.gongyeqx.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2470"}],"version-history":[{"count":1,"href":"http:\/\/www.gongyeqx.com\/index.php?rest_route=\/wp\/v2\/posts\/2470\/revisions"}],"predecessor-version":[{"id":2712,"href":"http:\/\/www.gongyeqx.com\/index.php?rest_route=\/wp\/v2\/posts\/2470\/revisions\/2712"}],"wp:attachment":[{"href":"http:\/\/www.gongyeqx.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2470"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.gongyeqx.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2470"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.gongyeqx.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2470"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}